{"id":59005,"date":"2024-05-07T14:57:13","date_gmt":"2024-05-07T12:57:13","guid":{"rendered":"https:\/\/lukasiewicz.gov.pl\/?p=59005"},"modified":"2024-05-07T15:01:31","modified_gmt":"2024-05-07T13:01:31","slug":"implementation-of-in-vitro-and-in-vivo-methods-for-the-assessment-of-endocrine-disrupting-properties","status":"publish","type":"post","link":"https:\/\/lukasiewicz.gov.pl\/en\/implementation-of-in-vitro-and-in-vivo-methods-for-the-assessment-of-endocrine-disrupting-properties\/","title":{"rendered":"Implementation of in vitro and in vivo methods for the assessment of endocrine disrupting properties"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"59005\" class=\"elementor elementor-59005\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d791abf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d791abf\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b1f4a7e\" data-id=\"b1f4a7e\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-500dfd5 elementor-widget elementor-widget-text-editor\" data-id=\"500dfd5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><b><span data-contrast=\"auto\">Authors\u00a0<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:288}\">\u00a0<\/span><\/h4><p><span data-contrast=\"auto\">Mateusz Paleczny, Natalia \u015alezi\u0144ska, Daniel Krakowian<br \/><\/span><span data-contrast=\"auto\">\u0141ukasiewicz Research Network &#8211; Institute of Industrial Organic Chemistry Branch Pszczyna<\/span>\u00a0<br \/><span data-contrast=\"auto\">e-mail: daniel.krakowian@ipo.lukasiewicz.gov.pl<\/span><span data-ccp-props=\"{&quot;134233117&quot;:false,&quot;134233118&quot;:false,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559738&quot;:0,&quot;335559739&quot;:0,&quot;335559740&quot;:288}\">\u00a0<\/span><\/p><h4><b><span data-contrast=\"auto\">Aim of the project<\/span><\/b><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/h4><p><span data-contrast=\"auto\">Methods for evaluating chemicals for endocrine disruption potential consist of\u202f<\/span><i><span data-contrast=\"auto\">in vitro<\/span><\/i><span data-contrast=\"auto\">\u202fand\u202f<\/span><i><span data-contrast=\"auto\">in vivo<\/span><\/i><span data-contrast=\"auto\">\u202ftests. The project aimed to implement methods to identify endocrine disruptors. The following test methods have been selected:<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/p><ul><li><span data-contrast=\"auto\"> The trans-activation assay of stably transfected human estrogen receptors for detecting estrogen agonist and antagonist actions using the hER<\/span><span data-contrast=\"auto\">\u03b1<\/span><span data-contrast=\"auto\">-HeLa-9903 (ERTA) cell line, according to the OECD guideline 455.<\/span><\/li><li><span data-contrast=\"auto\"> Uterotrophic Bioassay in Rodents: A short-term screening test for oestrogenic properties according to the OECD guideline 440.<\/span><\/li><li><span data-contrast=\"auto\"> Hershberger Bioassay in Rats: A Short-term Screening Assay for (Anti)Androgenic Properties, according to the OECD guideline 441).<\/span><\/li><\/ul><h4><b><span data-contrast=\"auto\">Short description of the problem addressed by this project<\/span><\/b><\/h4><p><span data-contrast=\"auto\">According to the WHO\/IPCS (2002) definition, an endocrine disruptor (ED) is an exogenous substance or mixture that affects the function of the endocrine system, producing adverse effects in a healthy organism and its progeny.\u202f<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/p><p><span data-contrast=\"auto\">The European Union agreed on scientific criteria for assessing substances for ED under the Plant Protection (EC 1107\/2009) and Biocidal Products (EU 528\/2012) Regulations. To classify a test substance as an ED, test results are required to establish a correlation between adverse effects and their causal relationship with exposure to the substance, indicating its impact on the endocrine system. In addition, ECHA\/EFSA guidance has been issued on the identification of EDs in the context of Regulations. Manufacturers of substances were required to identify the risks of possible endocrine-disrupting properties by the scientific criteria set out in the Commission Regulation (EU 2017\/2100 &#8211; biocidal products and 2018\/605 &#8211; plant protection products). Due to their serious risk to human and animal health, the identification of these substances is crucial during the registration process of chemicals and their mixtures, plant protection products, medicinal, veterinary, and biocidal products, feed\/food additives, industrial waste, household chemical products, and others.\u202f<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/p><h4><b><span data-contrast=\"auto\">Main results and achievements<\/span><\/b><\/h4><ul><li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"21\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;multilevel&quot;}\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">An\u202f<\/span><i><span data-contrast=\"auto\">in vitro<\/span><\/i><span data-contrast=\"auto\">\u202f(OECD 455) and\u202f<\/span><i><span data-contrast=\"auto\">in vivo<\/span><\/i><span data-contrast=\"auto\">\u202f(OECD 440\/441) test methods were validated.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/li><li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"21\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;multilevel&quot;}\" data-aria-posinset=\"2\" data-aria-level=\"1\"><span data-contrast=\"auto\">OECD 455: 14 reference substances (in 7 concentrations and three replicates) were tested for their effects on the estrogen receptor in the agonist test (substances stimulating the receptor), and 10 reference substances (in 6 concentrations and three replicates) in the antagonist test (substances inhibiting the stimulated receptor). At least two series were performed. All substances were correctly classified.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/li><li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"21\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;multilevel&quot;}\" data-aria-posinset=\"3\" data-aria-level=\"1\"><span data-contrast=\"auto\">OECD 440: five groups of female Wistar rats (6 in each group) were treated with different doses of the reference substance. A test system was obtained from parental pairs. Proficiency in the successful reproduction of rats was confirmed, and a method of marking young rats with a tattoo was developed. <\/span><span data-contrast=\"auto\">The results obtained were correct.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/li><li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"21\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;multilevel&quot;}\" data-aria-posinset=\"4\" data-aria-level=\"1\"><span data-contrast=\"auto\">OECD 441: three groups of male Wistar rats (6 in each group) were treated with two reference substances or vehicles alone. Androgen-dependent organs were excised and weighed. Proficiency in surgery and perioperative care, postoperative analgesia, and the impact of individual analgesics on animal welfare was acquired. The results obtained were correct. OECD 455, 440, and 410 methods were implemented for the \u0141ukasiewicz &#8211; IPO offering.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/li><\/ul><ul><li data-leveltext=\"\uf0b7\" data-font=\"Symbol\" data-listid=\"21\" data-list-defn-props=\"{&quot;335552541&quot;:1,&quot;335559685&quot;:720,&quot;335559991&quot;:360,&quot;469769226&quot;:&quot;Symbol&quot;,&quot;469769242&quot;:[8226],&quot;469777803&quot;:&quot;left&quot;,&quot;469777804&quot;:&quot;\uf0b7&quot;,&quot;469777815&quot;:&quot;multilevel&quot;}\" data-aria-posinset=\"1\" data-aria-level=\"1\"><span data-contrast=\"auto\">Historical data were obtained for each method.<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/li><\/ul><h4><b><span data-contrast=\"auto\">Conclusion<\/span><\/b><\/h4><p><span data-contrast=\"auto\">\u0141ukasiewicz \u2013 Institute of Industrial Organic Chemistry demonstrated proficiency in the use of research methods identifying substances that disrupt the functioning of the endocrine system. \u0141ukasiewicz \u2013 IPO offers domestic and foreign clients tests to evaluate chemicals for endocrine disorders through a multi-level testing strategy:<\/span><span data-ccp-props=\"{&quot;134233117&quot;:true,&quot;134233118&quot;:true,&quot;201341983&quot;:0,&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559739&quot;:280,&quot;335559740&quot;:288}\">\u00a0<\/span><\/p><ul><li><span data-contrast=\"auto\"> Level 1 -\u202f<\/span><i><span data-contrast=\"auto\">in silico<\/span><\/i><span data-contrast=\"auto\">\u202fmethods &#8211; collecting existing information about a given test material using computational methods, e.g., QSAR, predictive models.<\/span><\/li><li><span data-contrast=\"auto\"> Level 2 -\u202f<\/span><i><span data-contrast=\"auto\">in vitro<\/span><\/i><span data-contrast=\"auto\">\u202fmethods &#8211; providing information on selected endocrine mechanisms or pathways for a given test material (including OECD 455).<\/span><\/li><li><span data-contrast=\"auto\"> Level 3 -\u202f<\/span><i><span data-contrast=\"auto\">in vivo<\/span><\/i><span data-contrast=\"auto\">\u202fmethods &#8211; providing information on selected endocrine mechanisms or pathways for a given test material (OECD 440 and 441 methods).<\/span><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0dffcb8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0dffcb8\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0efba33\" data-id=\"0efba33\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cbb1c97 elementor-widget elementor-widget-gallery\" data-id=\"cbb1c97\" data-element_type=\"widget\" data-e-type=\"widget\" data-settings=\"{&quot;lazyload&quot;:&quot;yes&quot;,&quot;gallery_layout&quot;:&quot;grid&quot;,&quot;columns&quot;:4,&quot;columns_tablet&quot;:2,&quot;columns_mobile&quot;:1,&quot;gap&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;gap_tablet_extra&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;gap_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;gap_mobile_extra&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;gap_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;link_to&quot;:&quot;file&quot;,&quot;aspect_ratio&quot;:&quot;3:2&quot;,&quot;overlay_background&quot;:&quot;yes&quot;,&quot;content_hover_animation&quot;:&quot;fade-in&quot;}\" data-widget_type=\"gallery.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-gallery__container\">\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.2_in-vitro_in-vivo_L-IPO_O.Pszczyna-scaled.jpg\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"cbb1c97\" data-elementor-lightbox-title=\"\u0141-IPO_O.Pszczyna fot.2_in vitro_in vivo_\u0141-IPO_O.Pszczyna\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6NTkwMTEsInVybCI6Imh0dHBzOlwvXC9sdWthc2lld2ljei5nb3YucGxcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjRcLzA1XC9MLUlQT19PLlBzemN6eW5hLWZvdC4yX2luLXZpdHJvX2luLXZpdm9fTC1JUE9fTy5Qc3pjenluYS1zY2FsZWQuanBnIiwic2xpZGVzaG93IjoiY2JiMWM5NyJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.2_in-vitro_in-vivo_L-IPO_O.Pszczyna-265x300.jpg\" data-width=\"265\" data-height=\"300\" aria-label=\"\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.3_in-vitro_in-vivo.jpg\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"cbb1c97\" data-elementor-lightbox-title=\"\u0141-IPO_O.Pszczyna fot.3_in vitro_in vivo\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6NTkwMTQsInVybCI6Imh0dHBzOlwvXC9sdWthc2lld2ljei5nb3YucGxcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjRcLzA1XC9MLUlQT19PLlBzemN6eW5hLWZvdC4zX2luLXZpdHJvX2luLXZpdm8uanBnIiwic2xpZGVzaG93IjoiY2JiMWM5NyJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.3_in-vitro_in-vivo-300x200.jpg\" data-width=\"300\" data-height=\"200\" aria-label=\"\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.5_in-vitro_in-vivo.jpg\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"cbb1c97\" data-elementor-lightbox-title=\"\u0141-IPO_O.Pszczyna fot.5_in vitro_in vivo\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6NTkwMTgsInVybCI6Imh0dHBzOlwvXC9sdWthc2lld2ljei5nb3YucGxcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjRcLzA1XC9MLUlQT19PLlBzemN6eW5hLWZvdC41X2luLXZpdHJvX2luLXZpdm8uanBnIiwic2xpZGVzaG93IjoiY2JiMWM5NyJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.5_in-vitro_in-vivo-300x175.jpg\" data-width=\"300\" data-height=\"175\" aria-label=\"\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t<a class=\"e-gallery-item elementor-gallery-item elementor-animated-content\" href=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.6_in-vitro_in-vivo.jpg\" data-elementor-open-lightbox=\"yes\" data-elementor-lightbox-slideshow=\"cbb1c97\" data-elementor-lightbox-title=\"\u0141-IPO_O.Pszczyna fot.6_in vitro_in vivo\" data-e-action-hash=\"#elementor-action%3Aaction%3Dlightbox%26settings%3DeyJpZCI6NTkwMjAsInVybCI6Imh0dHBzOlwvXC9sdWthc2lld2ljei5nb3YucGxcL3dwLWNvbnRlbnRcL3VwbG9hZHNcLzIwMjRcLzA1XC9MLUlQT19PLlBzemN6eW5hLWZvdC42X2luLXZpdHJvX2luLXZpdm8uanBnIiwic2xpZGVzaG93IjoiY2JiMWM5NyJ9\">\n\t\t\t\t\t<div class=\"e-gallery-image elementor-gallery-item__image\" data-thumbnail=\"https:\/\/lukasiewicz.gov.pl\/wp-content\/uploads\/2024\/05\/L-IPO_O.Pszczyna-fot.6_in-vitro_in-vivo-265x300.jpg\" data-width=\"265\" data-height=\"300\" aria-label=\"\" role=\"img\" ><\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-gallery-item__overlay\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Authors\u00a0\u00a0 Mateusz Paleczny, Natalia \u015alezi\u0144ska, Daniel Krakowian\u0141ukasiewicz Research Network &#8211; Institute of Industrial Organic Chemistry Branch Pszczyna\u00a0e-mail: daniel.krakowian@ipo.lukasiewicz.gov.pl\u00a0 Aim of the project\u00a0 Methods for evaluating chemicals for endocrine disruption potential consist of\u202fin vitro\u202fand\u202fin vivo\u202ftests. The project aimed to implement methods to identify endocrine disruptors. The following test methods have been selected:\u00a0 The trans-activation assay of [&hellip;]<\/p>\n","protected":false},"author":140,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_seopress_robots_primary_cat":"none","_seopress_titles_title":"","_seopress_titles_desc":"","_seopress_robots_index":"yes","inline_featured_image":false,"footnotes":""},"categories":[519],"tags":[],"class_list":["post-59005","post","type-post","status-publish","format-standard","hentry","category-our-technologies-earto"],"acf":[],"publishpress_future_action":{"enabled":false,"date":"2026-04-30 05:14:25","action":"change-status","newStatus":"draft","terms":[],"taxonomy":"category","extraData":[]},"publishpress_future_workflow_manual_trigger":{"enabledWorkflows":[]},"_links":{"self":[{"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/posts\/59005","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/users\/140"}],"replies":[{"embeddable":true,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/comments?post=59005"}],"version-history":[{"count":4,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/posts\/59005\/revisions"}],"predecessor-version":[{"id":59024,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/posts\/59005\/revisions\/59024"}],"wp:attachment":[{"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/media?parent=59005"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/categories?post=59005"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lukasiewicz.gov.pl\/en\/wp-json\/wp\/v2\/tags?post=59005"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}